The input file can be of the following types:
  • Positional Nucleotide Numerical Summary (PNNS, BioEdit) data. Example
Reference: Nagy A, Jiřinec T, Černíková L, Jiřincová H, Havlíčková M. Large-scale nucleotide sequence alignment and sequence variability assessment to identify the evolutionarily highly conserved regions for universal screening PCR assay design: an example of influenza A virus.
Methods Mol Biol. 2015;1275: 57-72. doi: 10.1007/978-1-4939-2365-6_4.

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